Song Chen:Daily/NOTES/2014-2-21

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Hepatic commitment from high-density DE differentiation

Setting: Expose 1000 ms, PFBH 406, 10x obj,

AFP/HNF4A AFP/HNF6 AFP/PROX1
File:022114 H9DE AFP.png File:022114 H9DE AFP.2.png File:022114 H9DE AFP.3.png
File:022114 H9DE HNF4A.png File:022114 H9DE HNF6.png File:022114 H9DE PROX1.png

DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS

High seeding density: D6-D7 hES passage at 1:1.2-1:1.5 ratio

Successful hepatic differentiation of high-density DE differentiated cells


hES change medium:

  1. H9 p60 2*6 cm hES medium
  2. PGP1 iPS p30 1*6cm hES medium
  3. H9 p60 2*6 cm NHSM


Hepatic differentiation from PGP1 iPS:

16 well*48 well plate in DMEM/ITS


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