Song Chen:Daily/NOTES/2014-2-21
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Hepatic commitment from high-density DE differentiation
Setting: Expose 1000 ms, PFBH 406, 10x obj,
AFP/HNF4A | AFP/HNF6 | AFP/PROX1 |
File:022114 H9DE AFP.png | File:022114 H9DE AFP.2.png | File:022114 H9DE AFP.3.png |
File:022114 H9DE HNF4A.png | File:022114 H9DE HNF6.png | File:022114 H9DE PROX1.png |
DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS
High seeding density: D6-D7 hES passage at 1:1.2-1:1.5 ratio
Successful hepatic differentiation of high-density DE differentiated cells
hES change medium:
- H9 p60 2*6 cm hES medium
- PGP1 iPS p30 1*6cm hES medium
- H9 p60 2*6 cm NHSM
Hepatic differentiation from PGP1 iPS:
- 16 well*48 well plate in DMEM/ITS