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  • ==Design FISSEQ Padlock Probe Set for cDNA== *Design new probe set to target cDNA (- strand) instead of rolonies (+ strand) to skip low ef
    415 bytes (55 words) - 02:55, 18 September 2014
  • ==Design of padlock probes for the first proof-of-principle chip== *I will design padlock probes for both sets, then select a subset of 55k for the first chip. I wil
    1 KB (181 words) - 01:11, 16 November 2007
  • ===FISH with Padlock Probes II=== ===FISH with Padlock Probes===
    2 KB (273 words) - 23:53, 23 September 2014
  • *'''Padlock Probe Design for Baylor''' - Need to redo with hg19 =Padlock Probe Design for Baylor=
    976 bytes (133 words) - 23:38, 6 May 2011
  • == Plan for probe preparation for Matt's project == * Check reagents to make sure I have all reagents ready for probe prep and padlock capture
    1 KB (224 words) - 22:50, 6 March 2013
  • *ACTB target (on primary rolony) dye probe stock is almost gone so going to try same experiment with RAB7A **Considered using EGFR and other cancer genes but those padlock probe targets are thousands of kbp away from TSS which further complicates the pu
    892 bytes (143 words) - 01:42, 28 November 2013
  • ==Cancer Padlock Probes + RT Primer== *The cancer padlock probes from Illumina are actually made to capture cDNA, not primary rolonie
    4 KB (471 words) - 21:14, 20 March 2014
  • ==CES18bp probe design (Jan 09)== *Considerations of this probe set:
    4 KB (590 words) - 13:34, 16 July 2010
  • * Design LC Sciences Probes - need to analyze probes * Barcoding Padlock Probe libraries - talk to Dinh and Noi
    1 KB (174 words) - 22:38, 22 September 2011
  • ** '''Barcoding Padlock Probe libraries - Need to meet with Dinh and Noi''' ** Need to design primers that work on cDNA for these samples
    1 KB (209 words) - 00:43, 6 October 2011
  • * Design LC Sciences Probes - need to analyze probes * Barcoding Padlock Probe libraries - talk to Dinh and Noi
    723 bytes (115 words) - 23:53, 21 September 2011
  • * DONE - Design LC Sciences Probes - started design script * Barcoding Padlock Probe libraries - talk to Dinh and Noi
    2 KB (339 words) - 06:05, 21 September 2011
  • =Design and Order of Pull-down Probes= ...ay, amplify them using the same probe production protocol that we used for padlock probes (using the same adapter/primer arms), biotinylate the 3' end, and th
    6 KB (905 words) - 23:54, 22 February 2017
  • ==Design 2 Probes for FISH RCA Trial== **The padlock probes then capture to this rolony
    7 KB (830 words) - 01:26, 20 August 2013
  • ==Optimization of the padlock captur efficiency== The optimal dNTP0.08nM is for SNP probe with one base gap.
    5 KB (595 words) - 05:06, 18 March 2008
  • * '''DONE - Design LC Sciences Probes - sent probes to Dr. Zhang, Dinh, and Noi''' * Barcoding Padlock Probe libraries - talk to Dinh and Noi
    3 KB (453 words) - 00:51, 27 September 2011
  • =Target capture with padlock probes (designed 5/12) test= *Over the past few weeks, I've made ~1ug of padlock probes using Noi's probe production protocol (details to be posted soon)
    4 KB (455 words) - 19:51, 23 July 2014
  • =C Probe Design= ...ww.nature.com/nmeth/journal/v13/n3/abs/nmeth.3742.html Frei et al paper] C probe desing is based on
    5 KB (889 words) - 00:49, 22 April 2016
  • ...should get much better probe capture/more even bias compared to the larger probe set. *However, upon probe capture on new BA17 C1 single cell amplicons done on <http://genome-tech.uc
    5 KB (848 words) - 20:46, 2 July 2015
  • =Target capture with padlock probes (designed 5/12) test with Rui's probes as control (Part II - Capture *Have 13 rxn's (testing different probe:target ratios along with inclusion of more controls such as multiple negati
    4 KB (398 words) - 05:39, 20 August 2014
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