Tina:LabNotes/2012-5-25
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- Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well)
- Frozen down 3 vials of P6 InsACGGsAlov3 (from one 1:12 well and the remaining part of the passaged 1:12 well). Put cryovials at -80.
- Preparation of 0.1% gelatin solution
- Preparation of gelatin coated plates
- Seed MEF on gelatin coating plates (using MEF culture medium)