Tina:LabNotes/2012-6-1

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  • Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday).
  • Passage one well of 1:12 P8 InsACGGsAlov3 mESCs into 1:6 (1 well) and 1:3 (1 well; on 0.1% gelatin coating plate without MEF feeder cells; add MEF conditioned media)
  • Transfer 3 vials of P8 InsACGGsAlov3 from -80 to N2(l) tank.
  • Transfer all InsACGGsAlov3 c3lls into a new box (named "ZhangLab Box 3). Update cell storage information.
  • Transfer LIF from -80 to -20.