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New Insights Into The Metabolism Of Methyltestosterone And Metandienone: Detection Of Novel A-Ring Reduced Metabolites
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New Insights into the Metabolism of Methyltestosterone and Metandienone: Detection of Novel A-Ring Reduced Metabolites
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New Insights into the Metabolism of Methyltestosterone and Metandienone: Detection of Novel A-Ring Reduced Metabolites
Steffen Loke
Lingyu Liu
Maxi Wenzel
Heike Scheffler
Michele Iannone
Xavier de la Torre
Nils Schlörer
Francesco Botrè
Annekathrin Martina Keiler
Matthias Bureik
Maria Kristina Parr
Roles
Abstract
Introduction
Figure 1.
Figure 2.
Figure 3.
Results
1. Synthesis and Characterization of Reference Steroids
1.1. 17-Hydroxymethyl-17-methyl-18-nor-13-enes
1.2. 17β-Methyl-5β-androstane-3α,17α-diol
2. Post-Administration Urines
Table 3.
Figure 9.
Discussion
1. Chemical Syntheses and Characterization of Reference Material
2. Urinary Metabolites
Figure 10.
Figure 11.
Materials and Methods
1. Instrumentation
1.1. GC-MS/MS
1.2. GC-QTOF-MS
1.3. HPLC Purification
1.4. Nuclear Magnetic Resonance
2. Chemicals and Reagents
3. Synthesis of Reference Steroids
3.1. Diastereomeric 17-hydroxymethyl-17-methyl-18-nor-5-androst-13-en-3-ols
3.2. Epi-Tetrahydromethyltestosterones
4. Human Administration Trial
5. Urine Sample Preparation
Acknowledgments
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
Footnotes
References
Associated Data
Supplementary Materials
Data Availability Statement
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