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HESCs culture medium
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For 500 mL human H9 ES cell culture medium (H9 medium): {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Component''' | align="center" style="background:#f0f0f0;"|'''Volume''' | align="center" style="background:#f0f0f0;"|'''Final concentration''' |- | DMEM/F12||389.5 mL|| |- | KOSR||100 mL||20% |- | Non-essential amino acids (NEAA)||5 mL||0.1mM |- | Glutamax||5 mL||1mM |- |1000X BME soulution||500 uL||0.1mM |- | style="background:#f0f0f0;"|Total | style="background:#f0f0f0;"|500 mL | style="background:#f0f0f0;"| |} '''*Add FGF-2 and Y-27632(Rock inhibitor) freshly the day of use.''' *FGF-2: make 10 ug/ml stock (1:1000) aliquots, store at -20. *Y-27632: make 1 mM stock(1:200) aliquots, store at -20, once take out can leave at 4C for a while, avoid light. *Catalog number: DMEM/F12: Life Technologies 11330-032 KOSR: Life Technologies 10828-028 NEAA: Life Technologies 11140-050 Glutamax: Life Technologies 35050-061 BME solution: Life Technologies 21985-023
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