HESCs culture medium

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For 500 mL human H9 ES cell culture medium (H9 medium):

Component Volume Final concentration
DMEM/F12 389.5 mL
KOSR 100 mL 20%
Non-essential amino acids (NEAA) 5 mL 0.1mM
Glutamax 5 mL 1mM
1000X BME soulution 500 uL 0.1mM
Total 500 mL

*Add FGF-2 and Y-27632(Rock inhibitor) freshly the day of use.

  • FGF-2: make 10 ug/ml stock (1:1000) aliquots, store at -20.
  • Y-27632: make 1 mM stock(1:200) aliquots, store at -20, once take out can leave at 4C for a while, avoid light.


  • Catalog number:

DMEM/F12: Life Technologies 11330-032

KOSR: Life Technologies 10828-028

NEAA: Life Technologies 11140-050

Glutamax: Life Technologies 35050-061

BME solution: Life Technologies 21985-023