Tina:General cell culture protocols: Difference between revisions
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== | ==General ES cell culture== | ||
1. [[mouse ES cell culture medium]] | |||
2. [[mouse ES cell freezing medium]] | |||
3. [[human ES cell freezing medium]] | |||
==Feeder dependent ES cell culture== | |||
1. [[Preparation of 0.1% gelatin solution]] | |||
2. [[Preparation of gelatin coated plates]] | |||
3. [[MEF culture medium]] | |||
4. [[MEF conditioned medium]] | |||
5. [[Preparation of inactivated MEFs]] | |||
==Feeder independent hES cell culture (Matrigel)== | |||
1. [[Preparation of Matrigel plates]] | |||
2. [[Thawing hESCs on Matrigel plate]] | |||
3. [[Passaging hESCs on Matrigel plate (HUES)]] | |||
4. [[Passaging hESCs on Matrigel plate mechanically (H1, H9)]] | |||
4. | |||
Revision as of 00:58, 2 June 2012
General ES cell culture
1. mouse ES cell culture medium
2. mouse ES cell freezing medium
3. human ES cell freezing medium
Feeder dependent ES cell culture
1. Preparation of 0.1% gelatin solution
2. Preparation of gelatin coated plates
5. Preparation of inactivated MEFs
Feeder independent hES cell culture (Matrigel)
1. Preparation of Matrigel plates
2. Thawing hESCs on Matrigel plate
3. Passaging hESCs on Matrigel plate (HUES)
4. Passaging hESCs on Matrigel plate mechanically (H1, H9)
Osteogenic differentiation media
MEM containing 0-20% heat-inactivated ES-FBS (to be determined), 10−7 M Dexamethasone, 50 μg/ml Ascorbic acid and 10 mM β-glycerolphosphate