MESCs culture medium

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For 500 mL mouse ES cell culture medium (mESC medium):

Component Volume
Knockout DMEM 409.5 mL
ESC qualified FBS (heat inactivated) 75 mL
Non-essential amino acids (NEAA) 5 mL
Glutamax 5 mL
Penicillin / Streptomycin 5 mL
1000X BME soulution 500 uL
ESGRO LIF 50 uL
Total 500 mL



mESC medium preparation notes:[edit]

  • 5/7/2012

DMEM(Gibco); Hyclone characterized FBS (Hyclone); NEAA (Gibco); Glutamax (Gibco); Pen/Strep (Gibco); betamercaptomethanol; LIF (6 mL homemade LIF from Dr. Xu's Lab).

Note: mESC medium were prepared in Dr. Xu's Lab using Dr. Blue Lake's protocol.
  • 6/4/2012

KO DMEM(Gibco; cat#10829018; Lot#1094122; Exp 12/2012); ES-FBS (Gibco; cat#16141079; Exp 9/20/2015); NEAA (Gibco; cat#11140050; Lot#1105626; Exp 01/2013); Glutamax (Gibco; cat#35050061; Lot#1128305; Exp 02/2014); Pen/Strep (Gibco; cat#15140122; Exp 11/2011(expired)); BME (Gibco; cat#21985023; Lot#1134674; Exp 03/2015); LIF (homemade LIF from Dr. Xu's Lab).

Note: Instead of 50 uL ESGRO LIF, 6 mL of home made LIF was added.