HESCs culture medium: Difference between revisions
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>TinaLo (Created page with "For 500 mL human H9 ES cell culture medium (H9 medium): {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Component''' | align="center" style="background:#...") |
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'''*Add FGF-2 and Y-27632(Rock inhibitor) freshly the day of use.''' | |||
*FGF-2: make 10 ug/ml stock (1:1000) aliquots, store at -20. | |||
*Y-27632: make 1 mM stock(1:200) aliquots, store at -20, once take out can leave at 4C for a while, avoid light. | |||
Latest revision as of 22:39, 7 May 2013
For 500 mL human H9 ES cell culture medium (H9 medium):
Component | Volume | Final concentration |
DMEM/F12 | 389.5 mL | |
KOSR | 100 mL | 20% |
Non-essential amino acids (NEAA) | 5 mL | 0.1mM |
Glutamax | 5 mL | 1mM |
1000X BME soulution | 500 uL | 0.1mM |
Total | 500 mL |
*Add FGF-2 and Y-27632(Rock inhibitor) freshly the day of use.
- FGF-2: make 10 ug/ml stock (1:1000) aliquots, store at -20.
- Y-27632: make 1 mM stock(1:200) aliquots, store at -20, once take out can leave at 4C for a while, avoid light.
- Catalog number:
DMEM/F12: Life Technologies 11330-032
KOSR: Life Technologies 10828-028
NEAA: Life Technologies 11140-050
Glutamax: Life Technologies 35050-061
BME solution: Life Technologies 21985-023