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>Alan6017518 (Created page with '==Quantify libraries with Phi-X ladder qPCR== ==AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library== {| {{table}} | Setup|||||| |- |…') |
>Alan6017518 (→AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library) |
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[[Media:AL-Exome-CViB-Jan14-AB_2.pdf]] | [[Media:AL-Exome-CViB-Jan14-AB_2.pdf]] | ||
[[Media:AL-Exome-CViB-Jan14-CDE_2.pdf]] | [[Media:AL-Exome-CViB-Jan14-CDE_2.pdf]] | ||
==JD-cpg97k+30k-Breast_cancer-01230B09-Jan15== | |||
{| {{table}} | |||
| ||Well / Set||C(t)||||Difference||nM | |||
|- | |||
| PhiX||A1||6.98||7.06|||| | |||
|- | |||
| PhiX||B1||7.2|||||| | |||
|- | |||
| PhiX||C1||7.16|||||| | |||
|- | |||
| PhiX||D1||6.89|||||| | |||
|- | |||
| JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||E1||6.82||6.92||0.14||1.1 | |||
|- | |||
| JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||F1||7.09|||||| | |||
|- | |||
| JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||G1||6.89|||||| | |||
|- | |||
| JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||H1||6.86|||||| | |||
|- | |||
| | |||
|} | |||
==AL-Exome-CViB-Jan14-AB== | |||
{| {{table}} | |||
| ||Well / Set||C(t)||Ave.||Difference||nM | |||
|- | |||
| |||||||||| | |||
|- | |||
| |||||||||| | |||
|- | |||
| PhiX||A1||7.99||8.11|||| | |||
|- | |||
| PhiX||B1||8.33|||||| | |||
|- | |||
| PhiX||C1||8.18|||||| | |||
|- | |||
| PhiX||D1||7.95|||||| | |||
|- | |||
| AL-Exome-CViB-Jan14-AB||B2||4.48||4.51||3.61||12.17 | |||
|- | |||
| AL-Exome-CViB-Jan14-AB||C2||4.57|||||| | |||
|- | |||
| AL-Exome-CViB-Jan14-AB||D2||4.47|||||| | |||
|- | |||
| | |||
|} | |||
==1/10 AL-Exome-CViB-Jan14-AB== | |||
{| {{table}} | |||
| ||Well / Set||C(t)||Ave.||Difference||nM(X10DF.) | |||
|- | |||
| PhiX||A1||8.55||8.7|||| | |||
|- | |||
| PhiX||B1||8.96|||||| | |||
|- | |||
| PhiX||C1||8.74|||||| | |||
|- | |||
| PhiX||D1||8.56|||||| | |||
|- | |||
| 1/10 AL-Exome-CViB-Jan14-AB||G2||8.13||7.99||0.71||16.39 | |||
|- | |||
| 1/10 AL-Exome-CViB-Jan14-AB||H2||7.85 | |||
|- | |||
| | |||
|} | |||
==1/10 AL-Exome-CViB-Jan14-CDE== | |||
{| {{table}} | |||
| ||Well / Set||C(t)||Ave.||Difference||nM (X10DF.) | |||
|- | |||
| PhiX||A1||7.66||7.78|||| | |||
|- | |||
| PhiX||B1||8|||||| | |||
|- | |||
| PhiX||C1||7.86|||||| | |||
|- | |||
| PhiX||D1||7.61|||||| | |||
|- | |||
| 1/10 AL-Exome-CViB-Jan14-CDE||E3||6.57||6.68||1.1||21.44 | |||
|- | |||
| 1/10 AL-Exome-CViB-Jan14-CDE||F3||6.69|||||| | |||
|- | |||
| 1/10 AL-Exome-CViB-Jan14-CDE||G3||6.72|||||| | |||
|- | |||
| 1/10 AL-Exome-CViB-Jan14-CDE||H3||6.75|||||| | |||
|- | |||
| | |||
|} | |||
==AL-Exome-CViB-Jan14-CDE== | |||
{| {{table}} | |||
| ||Well / Set||C(t)||Ave.||Difference||nM | |||
|- | |||
| PhiX||A1||7.22||7.32|||| | |||
|- | |||
| PhiX||B1||7.49|||||| | |||
|- | |||
| PhiX||C1||7.41|||||| | |||
|- | |||
| PhiX||D1||7.16|||||| | |||
|- | |||
| AL-Exome-CViB-Jan14-CDE||A3||3.91||4.14||3.19||9.09 | |||
|- | |||
| AL-Exome-CViB-Jan14-CDE||B3||4.14|||||| | |||
|- | |||
| AL-Exome-CViB-Jan14-CDE||C3||4.25|||||| | |||
|- | |||
| AL-Exome-CViB-Jan14-CDE||D3||4.24|||||| | |||
|- | |||
| | |||
|} | |||
==Result== | |||
{| {{table}} | |||
| ||||nM | |||
|- | |||
| JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||||1.1 | |||
|- | |||
| 1/10 Ida|||| | |||
|- | |||
| AL-Exome-CViB-Jan14-AB||||12.17 | |||
|- | |||
| 1/10 AL-Exome-CViB-Jan14-AB||||16.39 | |||
|- | |||
| |||| | |||
|- | |||
| AL-Exome-CViB-Jan14-CDE||||9.09 | |||
|- | |||
| 1/10 AL-Exome-CViB-Jan14-CDE||||21.44 | |||
|- | |||
| | |||
|} |
Latest revision as of 22:41, 21 January 2010
Quantify libraries with Phi-X ladder qPCR[edit]
AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library[edit]
Setup | |||
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
For 2 wells | X17 | |
DNA | 2.000 | |
Syb_FP5 (100uM) | 0.200 | 3.400 |
Syb_RP7 (100uM) | 0.200 | 3.400 |
2X Phusion | 25.000 | 425.000 |
50X SYBR Green I | 0.200 | 3.400 |
H20 | 24.000 | 408.000 |
* Dilute sample, 1ul in 9ul ddh2o * mix 2ul 1/10 sample with 98ul mix in non-stick tube * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15_log_2.pdf Media:AL-Exome-CViB-Jan14-AB_2.pdf Media:AL-Exome-CViB-Jan14-CDE_2.pdf
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15[edit]
Well / Set | C(t) | Difference | nM | ||
PhiX | A1 | 6.98 | 7.06 | ||
PhiX | B1 | 7.2 | |||
PhiX | C1 | 7.16 | |||
PhiX | D1 | 6.89 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | E1 | 6.82 | 6.92 | 0.14 | 1.1 |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | F1 | 7.09 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | G1 | 6.89 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | H1 | 6.86 | |||
AL-Exome-CViB-Jan14-AB[edit]
Well / Set | C(t) | Ave. | Difference | nM | |
PhiX | A1 | 7.99 | 8.11 | ||
PhiX | B1 | 8.33 | |||
PhiX | C1 | 8.18 | |||
PhiX | D1 | 7.95 | |||
AL-Exome-CViB-Jan14-AB | B2 | 4.48 | 4.51 | 3.61 | 12.17 |
AL-Exome-CViB-Jan14-AB | C2 | 4.57 | |||
AL-Exome-CViB-Jan14-AB | D2 | 4.47 | |||
1/10 AL-Exome-CViB-Jan14-AB[edit]
Well / Set | C(t) | Ave. | Difference | nM(X10DF.) | |
PhiX | A1 | 8.55 | 8.7 | ||
PhiX | B1 | 8.96 | |||
PhiX | C1 | 8.74 | |||
PhiX | D1 | 8.56 | |||
1/10 AL-Exome-CViB-Jan14-AB | G2 | 8.13 | 7.99 | 0.71 | 16.39 |
1/10 AL-Exome-CViB-Jan14-AB | H2 | 7.85 | |||
1/10 AL-Exome-CViB-Jan14-CDE[edit]
Well / Set | C(t) | Ave. | Difference | nM (X10DF.) | |
PhiX | A1 | 7.66 | 7.78 | ||
PhiX | B1 | 8 | |||
PhiX | C1 | 7.86 | |||
PhiX | D1 | 7.61 | |||
1/10 AL-Exome-CViB-Jan14-CDE | E3 | 6.57 | 6.68 | 1.1 | 21.44 |
1/10 AL-Exome-CViB-Jan14-CDE | F3 | 6.69 | |||
1/10 AL-Exome-CViB-Jan14-CDE | G3 | 6.72 | |||
1/10 AL-Exome-CViB-Jan14-CDE | H3 | 6.75 | |||
AL-Exome-CViB-Jan14-CDE[edit]
Well / Set | C(t) | Ave. | Difference | nM | |
PhiX | A1 | 7.22 | 7.32 | ||
PhiX | B1 | 7.49 | |||
PhiX | C1 | 7.41 | |||
PhiX | D1 | 7.16 | |||
AL-Exome-CViB-Jan14-CDE | A3 | 3.91 | 4.14 | 3.19 | 9.09 |
AL-Exome-CViB-Jan14-CDE | B3 | 4.14 | |||
AL-Exome-CViB-Jan14-CDE | C3 | 4.25 | |||
AL-Exome-CViB-Jan14-CDE | D3 | 4.24 | |||
Result[edit]
nM | ||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1.1 | |
1/10 Ida | ||
AL-Exome-CViB-Jan14-AB | 12.17 | |
1/10 AL-Exome-CViB-Jan14-AB | 16.39 | |
AL-Exome-CViB-Jan14-CDE | 9.09 | |
1/10 AL-Exome-CViB-Jan14-CDE | 21.44 | |