2nd
Jump to navigation
Jump to search
Quantify libraries with Phi-X ladder qPCR[edit]
AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library[edit]
Setup | |||
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
For 2 wells | X17 | |
DNA | 2.000 | |
Syb_FP5 (100uM) | 0.200 | 3.400 |
Syb_RP7 (100uM) | 0.200 | 3.400 |
2X Phusion | 25.000 | 425.000 |
50X SYBR Green I | 0.200 | 3.400 |
H20 | 24.000 | 408.000 |
* Dilute sample, 1ul in 9ul ddh2o * mix 2ul 1/10 sample with 98ul mix in non-stick tube * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15_log_2.pdf Media:AL-Exome-CViB-Jan14-AB_2.pdf Media:AL-Exome-CViB-Jan14-CDE_2.pdf
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15[edit]
Well / Set | C(t) | Difference | nM | ||
PhiX | A1 | 6.98 | 7.06 | ||
PhiX | B1 | 7.2 | |||
PhiX | C1 | 7.16 | |||
PhiX | D1 | 6.89 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | E1 | 6.82 | 6.92 | 0.14 | 1.1 |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | F1 | 7.09 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | G1 | 6.89 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | H1 | 6.86 | |||
AL-Exome-CViB-Jan14-AB[edit]
Well / Set | C(t) | Ave. | Difference | nM | |
PhiX | A1 | 7.99 | 8.11 | ||
PhiX | B1 | 8.33 | |||
PhiX | C1 | 8.18 | |||
PhiX | D1 | 7.95 | |||
AL-Exome-CViB-Jan14-AB | B2 | 4.48 | 4.51 | 3.61 | 12.17 |
AL-Exome-CViB-Jan14-AB | C2 | 4.57 | |||
AL-Exome-CViB-Jan14-AB | D2 | 4.47 | |||
1/10 AL-Exome-CViB-Jan14-AB[edit]
Well / Set | C(t) | Ave. | Difference | nM(X10DF.) | |
PhiX | A1 | 8.55 | 8.7 | ||
PhiX | B1 | 8.96 | |||
PhiX | C1 | 8.74 | |||
PhiX | D1 | 8.56 | |||
1/10 AL-Exome-CViB-Jan14-AB | G2 | 8.13 | 7.99 | 0.71 | 16.39 |
1/10 AL-Exome-CViB-Jan14-AB | H2 | 7.85 | |||
1/10 AL-Exome-CViB-Jan14-CDE[edit]
Well / Set | C(t) | Ave. | Difference | nM (X10DF.) | |
PhiX | A1 | 7.66 | 7.78 | ||
PhiX | B1 | 8 | |||
PhiX | C1 | 7.86 | |||
PhiX | D1 | 7.61 | |||
1/10 AL-Exome-CViB-Jan14-CDE | E3 | 6.57 | 6.68 | 1.1 | 21.44 |
1/10 AL-Exome-CViB-Jan14-CDE | F3 | 6.69 | |||
1/10 AL-Exome-CViB-Jan14-CDE | G3 | 6.72 | |||
1/10 AL-Exome-CViB-Jan14-CDE | H3 | 6.75 | |||
AL-Exome-CViB-Jan14-CDE[edit]
Well / Set | C(t) | Ave. | Difference | nM | |
PhiX | A1 | 7.22 | 7.32 | ||
PhiX | B1 | 7.49 | |||
PhiX | C1 | 7.41 | |||
PhiX | D1 | 7.16 | |||
AL-Exome-CViB-Jan14-CDE | A3 | 3.91 | 4.14 | 3.19 | 9.09 |
AL-Exome-CViB-Jan14-CDE | B3 | 4.14 | |||
AL-Exome-CViB-Jan14-CDE | C3 | 4.25 | |||
AL-Exome-CViB-Jan14-CDE | D3 | 4.24 | |||
Result[edit]
nM | ||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1.1 | |
1/10 Ida | ||
AL-Exome-CViB-Jan14-AB | 12.17 | |
1/10 AL-Exome-CViB-Jan14-AB | 16.39 | |
AL-Exome-CViB-Jan14-CDE | 9.09 | |
1/10 AL-Exome-CViB-Jan14-CDE | 21.44 | |