CIRM BBAIII execution plan.: Difference between revisions

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==Year 1==
==Year 1==
*iPS reprogramming.
*iPSC reprogramming.
**Somatic cell types: Fibroblasts (PGP1F, BJ, IMR90), others??
**Somatic cell types: Fibroblasts (PGP1F, BJ, IMR90), others??
*hESC differentiation.
**Reprogramming methods: 4F, 4F plus POLR1C shRNA, episomal??
*hESC/hiPSC differentiation.
**H1 (need to obtain the cell line through MTA), Hues3, HUES8.
**H1 (need to obtain the cell line through MTA), Hues3, HUES8.
*Exome sequencing and mutation validation.
*Exome sequencing and mutation validation.

Latest revision as of 17:11, 27 June 2011

Year 1[edit]

  • iPSC reprogramming.
    • Somatic cell types: Fibroblasts (PGP1F, BJ, IMR90), others??
    • Reprogramming methods: 4F, 4F plus POLR1C shRNA, episomal??
  • hESC/hiPSC differentiation.
    • H1 (need to obtain the cell line through MTA), Hues3, HUES8.
  • Exome sequencing and mutation validation.
    • Existing lines (what??) versus new lines.
  • Gene knock-in/knock-down experiments.
    • What genes??
    • Build a database??