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Quantify libraries with Phi-X ladder qPCR
AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library
Setup | |||
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
Stock Phi-X (1nM) | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE | TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE | 1/10 TEST |
For 2 wells | X17 | |
DNA | 2.000 | |
Syb_FP5 (100uM) | 0.200 | 3.400 |
Syb_RP7 (100uM) | 0.200 | 3.400 |
2X Phusion | 25.000 | 425.000 |
50X SYBR Green I | 0.200 | 3.400 |
H20 | 24.000 | 408.000 |
* Dilute sample, 1ul in 9ul ddh2o * mix 2ul 1/10 sample with 98ul mix in non-stick tube * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15_log_2.pdf Media:AL-Exome-CViB-Jan14-AB_2.pdf Media:AL-Exome-CViB-Jan14-CDE_2.pdf