2nd

From ZhangLabWiki
Revision as of 21:49, 21 January 2010 by >Alan6017518 (Created page with '==Quantify libraries with Phi-X ladder qPCR== ==AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library== {| {{table}} | Setup|||||| |- |…')
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Quantify libraries with Phi-X ladder qPCR

AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library

Setup
Stock Phi-X (1nM) AL-Exome-CViB-Jan14-AB AL-Exome-CViB-Jan14-CDE TEST
Stock Phi-X (1nM) AL-Exome-CViB-Jan14-AB AL-Exome-CViB-Jan14-CDE TEST
Stock Phi-X (1nM) AL-Exome-CViB-Jan14-AB AL-Exome-CViB-Jan14-CDE TEST
Stock Phi-X (1nM) AL-Exome-CViB-Jan14-AB AL-Exome-CViB-Jan14-CDE TEST
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 1/10 AL-Exome-CViB-Jan14-AB 1/10 AL-Exome-CViB-Jan14-CDE 1/10 TEST
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 1/10 AL-Exome-CViB-Jan14-AB 1/10 AL-Exome-CViB-Jan14-CDE 1/10 TEST
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 1/10 AL-Exome-CViB-Jan14-AB 1/10 AL-Exome-CViB-Jan14-CDE 1/10 TEST
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 1/10 AL-Exome-CViB-Jan14-AB 1/10 AL-Exome-CViB-Jan14-CDE 1/10 TEST
For 2 wells X17
DNA 2.000
Syb_FP5 (100uM) 0.200 3.400
Syb_RP7 (100uM) 0.200 3.400
2X Phusion 25.000 425.000
50X SYBR Green I 0.200 3.400
H20 24.000 408.000
   * Dilute sample, 1ul in 9ul ddh2o
   * mix 2ul 1/10 sample with 98ul mix in non-stick tube
   * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)

98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min

Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15_log_2.pdf
Media:AL-Exome-CViB-Jan14-AB_2.pdf
Media:AL-Exome-CViB-Jan14-CDE_2.pdf