Revision as of 17:46, 6 May 2010 by >Zsakura2
August 14, 2009 (CV gDNA)
We have received Craig Venter's ips and fibroblast
ips line 1st elution: 314ng/ul
ips line 2nd elution: 127ng/ul (took out 30ul + 30ul ddH2O: 68ng/ul)
fibroblast 1st elution: 578ng/ul
fibroblast 2nd elution: 476.8ng/ul (took out 30ul + 30ul ddH2O: 272.9ng/ul)
January 27, 2010 (PGP1F cultured cell)
received 9 tubes of PGP1F cell pellet (100,000 cells each) from:
Jay Lee
WYSS Institute for Bio INS ENG
4 Blackfan Cir HIM 1040
Boston, MA 02115-5713
preformed DNA extraction on 1 tube [2-15-2010]
February 11 , 2010 (CVF gDNA from Goldstein lab)
picked up one tube of CVF (labeled as CVB, passage 13 fibroblast, 0.19ug/ul, ~100ul) from Jessica Young (Goldstein lab) in Leitchtag building
February 11, 2010 (gDNA from Eggan lab)
received 8 gDNA samples and 1 cell pellet from:
Evangelos Kiskinis
Harvard University
7 Divinity Avenue
Fairchild Biochemistry, Eggan Lab
Cambridge, MA 02138
cell type
|
Patient #
|
Line
|
Age/sex
|
genotype
|
Reprograming factor
|
Passage #
|
DNA (ng/ul)
|
hFib |
17 |
17 |
71/F |
WT |
- |
7 |
2000
|
ips |
17 |
17a |
71/F |
WT |
OCt4/Sox2/Klf4 |
20 |
447
|
ips |
17 |
17b |
71/F |
WT |
OCt4/Sox2/Klf4 |
23 |
2600
|
hFib |
11 |
11 |
36/M |
WT |
- |
7 |
2000
|
ips |
11 |
11a |
36/M |
WT |
OCt4/Sox2/Klf4 |
24 |
1037
|
ips |
11 |
11b |
36/M |
WT |
OCt4/Sox2/Klf4 |
20 |
1612
|
hFib |
29 |
29 |
29/F |
SOD1L144F |
- |
8 |
400
|
ips |
29 |
29e |
29/F |
SOD1L144F |
OCt4/Sox2/Klf4 |
21 |
cell pellet
|
ips |
29 |
29A |
29/F |
SOD1L144F |
Oct4/Sox2/Klf4/cMYC |
36 |
1200
|
|
preformed DNA extraction on ips 29e p21 [2-15-2010]
February 26, 2010 (sequencing libraries from Georgia)
- The samples were sent on 2/25/2010 through Fedex from:
Lynda Abernathy-Shi Lab
Medical College of Georgia
1120 15th Street
Augusta, GA 30912
Sample ID
|
Size
|
concentration (ng/ul)
|
Total Volume (ul)
|
EJ644 |
250-350bp |
10 |
18
|
EJ645 |
250-350bp |
10 |
15
|
EJ656 |
250-350bp |
3.6 |
17
|
EJ657 |
250-350bp |
3.2 |
20
|
|
|
March 2, 2010 (gDNA from Harvrd)
- received two tubes of sample from Jay Lee (harvard)
CD1 PGP1 ips P16: 399ng/ul x 200ul
PGP1F 80 gDNA: 299ng/ul x 200ul
March 4, 2010 (gDNA from Vanderbilt)
- received 10 samples from Vanderbilt Med Center
Joy Cogan, 615-322-7601
Vanderbilt Med Center
Div Medical Genetics DD2205 MCN
Nashville, TN 37232
- 3/3/2010 Vanderbilt IPF familial DNA samples
ID #
|
ng/ul
|
260/280
|
260/230
|
volume per tube
|
IPF 56JW525 |
65 |
1.86 |
1.38 |
80
|
IPF 56WW608 |
60 |
1.9 |
1.66 |
100
|
IPF 121BP1349 |
105 |
1.93 |
1.13 |
55
|
IPF 121DB1353 |
148 |
1.89 |
1.47 |
40
|
IPF 125DC1384 |
67 |
1.91 |
1.18 |
75
|
IPF 125GA1382 |
107 |
1.91 |
1.49 |
47
|
IPF 134GH1580 |
73 |
2.03 |
0.83 |
75
|
IPF 134LM1450 |
68 |
1.93 |
1.2 |
80
|
IPF 139JS1477 |
92 |
1.3 |
1.04 |
55
|
IPF 139TD1515 |
51 |
2.01 |
0.73 |
120
|
|
March 30, 2010 (more CV gDNA)
received 4 tubes of CV samples from Jessica Young(Goldstein lab): CV1, CV2, CV3, CV4
May 4, 2010 (gDNA from Salk)
Sergio Ruiz Macías, PhD
Gene Expression Lab (GEL-B)
Stem Cell Facility
The Salk Institute For Biological Studies
10010 North Torrey Pines Road
92037 La Jolla, CA, USA
sruiz@salk.edu
Tel. 858-453-4100, ext 1324
Fibroblast couple
|
concentration (ug/ul)
|
volume (ul)
|
passage number
|
HFF XF (Human Foreskin fibroblast Xeno free) |
0.2 |
50 |
9 |
|
FiPS 3F #1 |
0.178 |
56 |
24 |
|
|
|
|
|
keratinocyte couple |
|
|
|
Keratinocyte MMTA (human foreskin keratinocytes MMTA) |
0.5 |
20 |
5 |
|
KiPS 4F #8 |
0.42 |
23.8 |
28 |
|
|
|
|
|
Astrocytes |
|
|
|
human astrocytes |
0.36 |
90 |
12 |
|
ASTiPS4F-4 |
0.51 |
90 |
10 |
|
ASTiPS4F-5 |
0.71 |
90 |
10 |
|
|
|
|
|
HUVEC |
|
|
|
Human HUVEC |
1.12 |
90 |
2
|
HUViPS4F-1 |
1.03 |
90 |
10
|
HUViPS4F-3 |
0.47 |
90 |
15
|
|
- genomic DNA was extracted using the QIAmp DNA Mini and Blood Extraction Kit from Qiagen. DNA is resuspended in AE buffer (10mM Tris Cl; 0.5mM EDTA)
- priority:
- Keratinocyte MMTA and KiPS 4F #8
- Human HUVEC, HUViPS4F-1, HUViPS4F-3
- HFF XF, FiPS 3F #1
- human astrocytes, ASTiPS4F-4, ASTiPS4F-5