picked up one tube of CVF (labeled as CVB, passage 13 fibroblast, 0.19ug/ul, ~100ul) from Jessica Young (Goldstein lab) in Leitchtag building
February 11, 2010 (gDNA from Eggan lab)
received 8 gDNA samples and 1 cell pellet from:
Evangelos Kiskinis
Harvard University
7 Divinity Avenue
Fairchild Biochemistry, Eggan Lab
Cambridge, MA 02138
cell type
Patient #
Line
Age/sex
genotype
Reprograming factor
Passage #
DNA (ng/ul)
hFib
17
17
71/F
WT
-
7
2000
ips
17
17a
71/F
WT
OCt4/Sox2/Klf4
20
447
ips
17
17b
71/F
WT
OCt4/Sox2/Klf4
23
2600
hFib
11
11
36/M
WT
-
7
2000
ips
11
11a
36/M
WT
OCt4/Sox2/Klf4
24
1037
ips
11
11b
36/M
WT
OCt4/Sox2/Klf4
20
1612
hFib
29
29
29/F
SOD1L144F
-
8
400
ips
29
29e
29/F
SOD1L144F
OCt4/Sox2/Klf4
21
cell pellet
ips
29
29A
29/F
SOD1L144F
Oct4/Sox2/Klf4/cMYC
36
1200
preformed DNA extraction on ips 29e p21 [2-15-2010]
February 26, 2010 (sequencing libraries from Georgia)
The samples were sent on 2/25/2010 through Fedex from:
Lynda Abernathy-Shi Lab
Medical College of Georgia
1120 15th Street
Augusta, GA 30912
Sample ID
Size
concentration (ng/ul)
Total Volume (ul)
EJ644
250-350bp
10
18
EJ645
250-350bp
10
15
EJ656
250-350bp
3.6
17
EJ657
250-350bp
3.2
20
March 2, 2010 (gDNA from Harvrd)
received two tubes of sample from Jay Lee (harvard):
CD1 PGP1 ips P16: 399ng/ul x 200ul
PGP1F 80 gDNA: 299ng/ul x 200ul
March 4, 2010 (gDNA from Vanderbilt)
received 10 samples from Vanderbilt Med Center:
Joy Cogan, 615-322-7601
Vanderbilt Med Center
Div Medical Genetics DD2205 MCN
Nashville, TN 37232
3/3/2010 Vanderbilt IPF familial DNA samples
ID #
ng/ul
260/280
260/230
volume per tube
IPF 56JW525
65
1.86
1.38
80
IPF 56WW608
60
1.9
1.66
100
IPF 121BP1349
105
1.93
1.13
55
IPF 121DB1353
148
1.89
1.47
40
IPF 125DC1384
67
1.91
1.18
75
IPF 125GA1382
107
1.91
1.49
47
IPF 134GH1580
73
2.03
0.83
75
IPF 134LM1450
68
1.93
1.2
80
IPF 139JS1477
92
1.3
1.04
55
IPF 139TD1515
51
2.01
0.73
120
March 30, 2010 (more CV gDNA)
received 4 tubes of CV samples from Jessica Young(Goldstein lab): CV1, CV2, CV3, CV4
May 4, 2010 (gDNA from Salk)
Sergio Ruiz Macías, PhD
Gene Expression Lab (GEL-B)
Stem Cell Facility
The Salk Institute For Biological Studies
10010 North Torrey Pines Road
92037 La Jolla, CA, USA
sruiz@salk.edu
Tel. 858-453-4100, ext 1324