Revision as of 22:41, 21 January 2010 by >Alan6017518
Quantify libraries with Phi-X ladder qPCR[edit]
AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library[edit]
Setup |
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Stock Phi-X (1nM) |
AL-Exome-CViB-Jan14-AB |
AL-Exome-CViB-Jan14-CDE |
TEST
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Stock Phi-X (1nM) |
AL-Exome-CViB-Jan14-AB |
AL-Exome-CViB-Jan14-CDE |
TEST
|
Stock Phi-X (1nM) |
AL-Exome-CViB-Jan14-AB |
AL-Exome-CViB-Jan14-CDE |
TEST
|
Stock Phi-X (1nM) |
AL-Exome-CViB-Jan14-AB |
AL-Exome-CViB-Jan14-CDE |
TEST
|
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 |
1/10 AL-Exome-CViB-Jan14-AB |
1/10 AL-Exome-CViB-Jan14-CDE |
1/10 TEST
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JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 |
1/10 AL-Exome-CViB-Jan14-AB |
1/10 AL-Exome-CViB-Jan14-CDE |
1/10 TEST
|
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 |
1/10 AL-Exome-CViB-Jan14-AB |
1/10 AL-Exome-CViB-Jan14-CDE |
1/10 TEST
|
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 |
1/10 AL-Exome-CViB-Jan14-AB |
1/10 AL-Exome-CViB-Jan14-CDE |
1/10 TEST
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For 2 wells |
X17
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DNA |
2.000 |
|
Syb_FP5 (100uM) |
0.200 |
3.400
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Syb_RP7 (100uM) |
0.200 |
3.400
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2X Phusion |
25.000 |
425.000
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50X SYBR Green I |
0.200 |
3.400
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H20 |
24.000 |
408.000
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* Dilute sample, 1ul in 9ul ddh2o
* mix 2ul 1/10 sample with 98ul mix in non-stick tube
* aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15_log_2.pdf
Media:AL-Exome-CViB-Jan14-AB_2.pdf
Media:AL-Exome-CViB-Jan14-CDE_2.pdf
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15[edit]
|
Well / Set |
C(t) |
|
Difference |
nM
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PhiX |
A1 |
6.98 |
7.06 |
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PhiX |
B1 |
7.2 |
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PhiX |
C1 |
7.16 |
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PhiX |
D1 |
6.89 |
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JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 |
E1 |
6.82 |
6.92 |
0.14 |
1.1
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JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 |
F1 |
7.09 |
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JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 |
G1 |
6.89 |
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JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 |
H1 |
6.86 |
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AL-Exome-CViB-Jan14-AB[edit]
|
Well / Set |
C(t) |
Ave. |
Difference |
nM
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PhiX |
A1 |
7.99 |
8.11 |
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PhiX |
B1 |
8.33 |
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PhiX |
C1 |
8.18 |
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PhiX |
D1 |
7.95 |
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AL-Exome-CViB-Jan14-AB |
B2 |
4.48 |
4.51 |
3.61 |
12.17
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AL-Exome-CViB-Jan14-AB |
C2 |
4.57 |
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AL-Exome-CViB-Jan14-AB |
D2 |
4.47 |
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1/10 AL-Exome-CViB-Jan14-AB[edit]
|
Well / Set |
C(t) |
Ave. |
Difference |
nM(X10DF.)
|
PhiX |
A1 |
8.55 |
8.7 |
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|
PhiX |
B1 |
8.96 |
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PhiX |
C1 |
8.74 |
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PhiX |
D1 |
8.56 |
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1/10 AL-Exome-CViB-Jan14-AB |
G2 |
8.13 |
7.99 |
0.71 |
16.39
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1/10 AL-Exome-CViB-Jan14-AB |
H2 |
7.85
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|
1/10 AL-Exome-CViB-Jan14-CDE[edit]
|
Well / Set |
C(t) |
Ave. |
Difference |
nM (X10DF.)
|
PhiX |
A1 |
7.66 |
7.78 |
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PhiX |
B1 |
8 |
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PhiX |
C1 |
7.86 |
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PhiX |
D1 |
7.61 |
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1/10 AL-Exome-CViB-Jan14-CDE |
E3 |
6.57 |
6.68 |
1.1 |
21.44
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1/10 AL-Exome-CViB-Jan14-CDE |
F3 |
6.69 |
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1/10 AL-Exome-CViB-Jan14-CDE |
G3 |
6.72 |
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1/10 AL-Exome-CViB-Jan14-CDE |
H3 |
6.75 |
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AL-Exome-CViB-Jan14-CDE[edit]
|
Well / Set |
C(t) |
Ave. |
Difference |
nM
|
PhiX |
A1 |
7.22 |
7.32 |
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|
PhiX |
B1 |
7.49 |
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PhiX |
C1 |
7.41 |
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PhiX |
D1 |
7.16 |
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AL-Exome-CViB-Jan14-CDE |
A3 |
3.91 |
4.14 |
3.19 |
9.09
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AL-Exome-CViB-Jan14-CDE |
B3 |
4.14 |
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AL-Exome-CViB-Jan14-CDE |
C3 |
4.25 |
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AL-Exome-CViB-Jan14-CDE |
D3 |
4.24 |
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nM
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JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 |
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1.1
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1/10 Ida |
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AL-Exome-CViB-Jan14-AB |
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12.17
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1/10 AL-Exome-CViB-Jan14-AB |
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16.39
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AL-Exome-CViB-Jan14-CDE |
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9.09
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1/10 AL-Exome-CViB-Jan14-CDE |
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21.44
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