HESCs culture medium
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For 500 mL human H9 ES cell culture medium (H9 medium):
Component | Volume | Final concentration |
DMEM/F12 | 389.5 mL | |
KOSR | 100 mL | 20% |
Non-essential amino acids (NEAA) | 5 mL | 0.1mM |
Glutamax | 5 mL | 1mM |
1000X BME soulution | 500 uL | 0.1mM |
Total | 500 mL |
- Catalog number:
DMEM/F12: Life Technologies 11330-032
KOSR: Life Technologies 10828-028
NEAA: Life Technologies 11140-050
Glutamax: Life Technologies 35050-061
BME solution: Life Technologies 21985-023