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Quantify libraries with Phi-X ladder qPCR[edit]

AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library[edit]

Setup
1nm PhiX AL-Exome-CViB-Jan14-AB AL-Exome-CViB-Jan14-CDE
1nm PhiX AL-Exome-CViB-Jan14-AB AL-Exome-CViB-Jan14-CDE
1nm PhiX AL-Exome-CViB-Jan14-AB AL-Exome-CViB-Jan14-CDE
1nm PhiX AL-Exome-CViB-Jan14-AB AL-Exome-CViB-Jan14-CDE
Ida 1/10 AL-Exome-CViB-Jan14-AB 1/10 AL-Exome-CViB-Jan14-CDE
Ida 1/10 AL-Exome-CViB-Jan14-AB 1/10 AL-Exome-CViB-Jan14-CDE
Ida 1/10 AL-Exome-CViB-Jan14-AB 1/10 AL-Exome-CViB-Jan14-CDE
Ida 1/10 AL-Exome-CViB-Jan14-AB 1/10 AL-Exome-CViB-Jan14-CDE
2 Wells X13
DNA 2 26
Syb_FP5 (100uM) 0.2 2.6
Syb_RP7 (100uM) 0.2 2.6
2X Phusion 25 ,
50X SYBR Green 0.2 2.6
H2O 24 312
Total 345.8
   * Dilute sample, 1ul in 9ul ddh2o
   * mix 2ul 1/10 sample with 98ul mix in non-stick tube
   * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)

98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min

Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15_log.pdf
Media:AL-Exome-CViB-Jan14-AB.pdf
Media:AL-Exome-CViB-Jan14-CDE.pdf
C(t) @0.025 Average
PhiX A1 8.830 8.826 8.630
PhiX B1 8.630 8.634
PhiX D1 8.430 8.429
Ida E1 8.260 8.257 Average Difference nM
Ida F1 8.500 8.496 8.277 0.353 1.277
Ida G1 8.070 8.073
Ida H1 8.280 8.282
1/10 AL-Exome-CViB-Jan14-AB E2 7.290 7.289 Average Difference nM X10
1/10 AL-Exome-CViB-Jan14-AB F2 7.430 7.433 7.173 1.457 2.745 27.45
1/10 AL-Exome-CViB-Jan14-AB G2 6.950 6.953
1/10 AL-Exome-CViB-Jan14-AB H2 7.020 7.017
C(t) @0.015 Average
PhiX A1 7.640 7.642 7.505
PhiX B1 7.520 7.517
PhiX D1 7.350 7.355
AL-Exome-CViB-Jan14-AB A2 4.290 4.292 Average Difference nM
AL-Exome-CViB-Jan14-AB B2 4.260 4.261 4.306 3.199 9.181
AL-Exome-CViB-Jan14-AB C2 4.360 4.365
C(t) Average
PhiX A1 7.640 7.5
PhiX B1 7.520
PhiX D1 7.350
AL-Exome-CViB-Jan14-CDE A3 4.050 Average Difference nM
AL-Exome-CViB-Jan14-CDE B3 3.810 3.99 3.51 11.42
AL-Exome-CViB-Jan14-CDE C3 4.080
AL-Exome-CViB-Jan14-CDE D3 4.020
1/10 AL-Exome-CViB-Jan14-CDE E3 6.000 Average Difference nM X10
1/10 AL-Exome-CViB-Jan14-CDE F3 6.130 5.92 1.59 3.01 30.070
1/10 AL-Exome-CViB-Jan14-CDE G3 5.850
1/10 AL-Exome-CViB-Jan14-CDE H3 5.680