Cryopreservation of ESCs
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1. Detach cells as passaging.
2. Transfer the detached cells to a conical tube.
3. Rinse the wells with additional 2ml PBS. Add the rinse to the tube.
4. Cfg at 200g, 5 min.
5. Aspirate the supernatant, resuspend cells with cryopreservation media, and transfer to a cryovial.