Cryopreservation of ESCs

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1. Detach cells as passaging.

2. Transfer the detached cells to a conical tube.

3. Rinse the wells with additional 2ml PBS. Add the rinse to the tube.

4. Cfg at 200g, 5 min.

5. Aspirate the supernatant, resuspend cells with cryopreservation media, and transfer to a cryovial.