MESCs culture medium
Jump to navigation
Jump to search
For 500 mL mouse ES cell culture medium (mESC medium):
Component | Volume |
Knockout DMEM | 409.5 mL |
ESC qualified FBS (heat inactivated) | 75 mL |
Non-essential amino acids (NEAA) | 5 mL |
Glutamax | 5 mL |
Penicillin / Streptomycin | 5 mL |
1000X BME soulution | 500 uL |
ESGRO LIF | 50 uL |
Total | 500 mL |
mESC medium preparation notes:[edit]
- 5/7/2012
DMEM(Gibco); Hyclone characterized FBS (Hyclone); NEAA (Gibco); Glutamax (Gibco); Pen/Strep (Gibco); betamercaptomethanol; LIF (6 mL homemade LIF from Dr. Xu's Lab).
Note: mESC medium were prepared in Dr. Xu's Lab using Dr. Blue Lake's protocol.
- 6/4/2012
KO DMEM(Gibco; cat#10829018; Lot#1094122; Exp 12/2012); ES-FBS (Gibco; cat#16141079; Exp 9/20/2015); NEAA (Gibco; cat#11140050; Lot#1105626; Exp 01/2013); Glutamax (Gibco; cat#35050061; Lot#1128305; Exp 02/2014); Pen/Strep (Gibco; cat#15140122; Exp 11/2011(expired)); BME (Gibco; cat#21985023; Lot#1134674; Exp 03/2015); LIF (homemade LIF from Dr. Xu's Lab).
Note: Instead of 50 uL ESGRO LIF, 6 mL of home made LIF was added.