Thawing hESCs on feeder
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1. Thaw 1 vial of hESCs in waterbath
2. Transfer cells to a conical tube
3. Add 9ml warm culture medium dropwise to the tube, gently mixing as the medium is added.
4. Cfg at 200g, 5 min.
5. Aspirate the supernatant, and resuspend cells with culture medium, pipette as cell type suggested.
6. Transfer cells to culture plate/dish.