Thawing mESCs on feeder

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1. Thaw 1 vial of mESCs in waterbath

2. Transfer cells to a conical tube

3. Add 9ml culture medium dropwise to the tube, gently mixing as the medium is added.

4. Cfg at 200g, 5 min.

5. Aspirate the supernatant, and resuspend cells with culture medium, pipette to make a single cell suspension (do it gently, because the membranes are weak at this point).

6. Transfer cells to culture plate/dish.