Calendar/2010-1-16: Difference between revisions

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==Quantify libraries with Phi-X ladder qPCR==
[[1st]]
==AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library==
[[2nd]]
{| {{table}}
  [[3rd]]
| Setup||||
|-
| 1nm PhiX||AL-Exome-CViB-Jan14-AB ||AL-Exome-CViB-Jan14-CDE
|-
| 1nm PhiX||AL-Exome-CViB-Jan14-AB ||AL-Exome-CViB-Jan14-CDE
|-
| 1nm PhiX||AL-Exome-CViB-Jan14-AB ||AL-Exome-CViB-Jan14-CDE
|-
| 1nm PhiX||AL-Exome-CViB-Jan14-AB ||AL-Exome-CViB-Jan14-CDE
|-
| Ida||1/10 AL-Exome-CViB-Jan14-AB ||1/10 AL-Exome-CViB-Jan14-CDE
|-
| Ida||1/10 AL-Exome-CViB-Jan14-AB ||1/10 AL-Exome-CViB-Jan14-CDE
|-
| Ida||1/10 AL-Exome-CViB-Jan14-AB ||1/10 AL-Exome-CViB-Jan14-CDE
|-
| Ida||1/10 AL-Exome-CViB-Jan14-AB ||1/10 AL-Exome-CViB-Jan14-CDE
|-
|
|}
 
{| {{table}}
| ||2 Wells||X13
|-
| DNA||2||26
|-
| Syb_FP5 (100uM)||0.2||2.6
|-
| Syb_RP7 (100uM)||0.2||2.6
|-
| 2X Phusion||25|| ,
|-
| 50X SYBR Green||0.2||2.6
|-
| H2O||24||312
|-
| Total||||345.8
|-
|
|}
 
    * Dilute sample, 1ul in 9ul ddh2o
    * mix 2ul 1/10 sample with 98ul mix in non-stick tube
    * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)
 
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
  [[Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15.pdf]]

Latest revision as of 21:43, 21 January 2010