Tina:General cell culture protocols
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General ES cell culture medium
- mouse ESCs
- human ESCs
Feeder dependent ESCs culture protocol
- Coating
1. Preparation of gelatin coated plates
- MEF
3. Preparation of inactivated MEFs
- mouse ESCs
- human ESCs
Feeder independent ESCs culture protocol(Matrigel)
1. Preparation of Matrigel plates
2. Preparation of MEF conditioned medium
3. Thawing hESCs on Matrigel plate
4. Passaging hESCs on Matrigel plate (HUES)
5. Passaging hESCs on Matrigel plate mechanically (H1, H9)
Osteogenic differentiation media
MEM containing 0-20% heat-inactivated ES-FBS (to be determined), 10−7 M Dexamethasone, 50 μg/ml Ascorbic acid and 10 mM β-glycerolphosphate