Tina:General cell culture protocols: Difference between revisions
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6. [[Thawing hESCs on feeder]] | 6. [[Thawing hESCs on feeder]] | ||
7. [[Passaging hESCs on feeder]] | |||
*'''Cryopreservation''' | |||
8. [[Cryopreservation of ESCs]] | |||
==Feeder independent ESCs culture protocol(Matrigel)== | ==Feeder independent ESCs culture protocol(Matrigel)== |
Latest revision as of 22:27, 7 May 2013
General ES cell culture medium[edit]
- mouse ESCs
- human ESCs
Feeder dependent ESCs culture protocol[edit]
- Coating
1. Preparation of gelatin coated plates
- MEF
3. Preparation of inactivated MEFs
- mouse ESCs
- human ESCs
- Cryopreservation
Feeder independent ESCs culture protocol(Matrigel)[edit]
1. Preparation of Matrigel plates
2. Preparation of MEF conditioned medium
3. Thawing hESCs on Matrigel plate
4. Passaging hESCs on Matrigel plate (HUES)
5. Passaging hESCs on Matrigel plate mechanically (H1, H9)
Osteogenic differentiation media[edit]
MEM containing 0-20% heat-inactivated ES-FBS (to be determined), 10−7 M Dexamethasone, 50 μg/ml Ascorbic acid and 10 mM β-glycerolphosphate